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KMID : 0364819910290040209
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Korean Journal of Microbiology
1991 Volume.29 No. 4 p.209 ~ p.214
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Determination of Active Site in PRD1 DNA Polymerase by Site-specific Mutagenesis
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Abstract
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1
The PRD1 DNA polymerase is a small multi-functional enzyme containing conserved amino acid sequences shared by family B DNA polymerises. Thus the PRD1 DNA polymerise provides an useful model system with which to study structure-functional relationships of DNA polymerase molecules. In order to investigate the functional and structural roles of the highly conserved amino acid sequences, we have introduced three mutations into a conserved amino acid of the PRD1 DNA polymerase. Genetic complementation study indicated that each mutation inactivated DNA polymerise catalytic activity.´
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KEYWORD
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